What quality problems will occur with nucleic acid extraction instruments?
Since a complete PCR detection requires "two processes" of nucleic acid preparation and PCR amplification, there is no fully automatic nucleic acid detection system. With the improvement of the degree of automation and intelligence of nucleic acid extraction systems, instrumented nucleic acid preparation methods have been gradually applied in the clinical detection process, and there is a tendency to replace manual labor.
But the nucleic acid preparation of the instrument also has its shortcomings and deficiencies. This requires users to have a clear understanding of key links and formulate corresponding countermeasures, which is an important help to comprehensively improve the quality of testing.
1. Influence of sampling sensing function on quality
In the automatic sample injection nucleic acid extraction system, although some systems have better injection monitoring function and higher injection accuracy, some nucleic acid extraction systems use non-inductive tips or the quality of induction tips is different, and Occasionally, a failed induction can result in insufficient or no injection. This is especially important for systems that use deep-well plates for nucleic acid preparation. Users need to strengthen the maintenance and maintenance of the instrument to keep the instrument in normal operation. Additionally, test results should be continuously tracked. Once the feedback results are found to be inaccurate, the reasons should be promptly investigated.
2. Differences in the accuracy of the mechanical operation of the instrument
In terms of robotic arm movement and grasping, the accuracy of different nucleic acid extraction systems varies greatly. For the automatic nucleic acid extraction system with a high degree of intelligence, there is an immediate shutdown alarm function when the robotic arm fails, which affects the workflow but does not affect the detection quality. However, some instruments do not have robotic arm failure detection and alarm functions. For example, some nucleic acid extraction systems can remove the deep well plate and continue working. Such instruments require the preparation of nucleic acids under the supervision of the experimental staff in order to detect problems in a timely manner. Therefore, "instrument automation" is not "artificial intelligence", and human responsibility cannot be ignored,
3. Contamination sources of nucleic acid preparations for instruments
Long-term practical experience has proved that naturally evaporated aerosols are not the main cause of PCR detection contamination, but carrying cross-contamination is the culprit! Even COBAS using SPU units for nucleic acid preparation cannot avoid cross-contamination caused by bubble bursting, but the probability is low. However, for instruments that use 96-well deep-well plates for nucleic acid preparation, it is difficult to avoid cross-contamination due to the power supply, heating, elution and other links between dense wells.
Therefore, laboratory contamination monitoring of instrument nucleic acid preparations is very necessary! Especially in infectious disease hospitals, most of their samples are from nucleic acid-positive patients. Monitoring needs to set up multiple negative controls, but it is difficult to achieve in clinical work, with high cost and trouble.
We can clean instruments and laboratory tabletops after experiments with nucleic acid scavengers.
Geneture, a one-stop solution for nucleic acid extraction and analysis. If you need more information about nucleic acid extraction instruments, please feel free to contact.
Geneture, a professional manufacturer of nucleic acid (DNA & RNA) extraction kits and analytical products
GENETURE is a group company,we own two factories: Ascend and Dianrun,to provide one stop solution of Nucleic Acid Extraction and Analysis,including solution for COVID-19. Geneture provides high quality and professional Nucleic Acid Extraction Reagents, Lab consumables, Real-time PCR consumables and test machines.
The kit uses magnetic beads to adsorb DNA/RNA to achieve the purpose of rapid purification of DNA/RNA. It is suitable for extracting high-purity DNA/RNA from samples (grinding supernatant of animal and plant tissues, whole blood, serum, plasma, cell culture supernatant, oral and throat swabs, body fluids, genital secretions, bacteria and viruses, etc.) For molecular biology experiments such as PCR/RT-PCR, Real-time PCR/Real-time RT-PCR, etc. If you need more information, please feel free to contact us.
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